prevalence of clostridium botulinum type a, b, e and f isolated from directly sold honey in lithuania
نویسندگان
چکیده
the aim of the study was the analysis of lithuanian honeys for the presence of the spores of clostridium botulinum. the analyses were performed on multifloral, honeydew, nectar-honeydew, rape, buckwheat, heather and linden honey bought in direct sale. prior to culture on solid media the samples were prepared by supernatant filtration method and cultured in parallel in cooked meat medium (cmm) and tripticase peptone glucose yeast (tpgy) enrichment broths. bacteria of typical phenotypic features were obtained by culture method from 48 honey samples. identification of clostridium botulinum types a, b, e and f was performed with the use of multiplex-pcr method. the prevalence analysis showed 30 (60%) samples contaminated with clostrdium botulinum spores. the major serotype detected was type a: 20 (66.6%) positive honey samples; while type b was found in 7 samples (23.3%), type e in 4 samples (13.3%) and type f in 3 (10%) positive honey samples. contamination with more than one type of c. botulinum spores was observed in 3 honey samples.
منابع مشابه
Characterization of Clostridium botulinum spores and its toxin in honey
Botulism is a serious paralytic disease caused by Clostridium botulinum toxin in foods. There are seven recognized serotypes of botulinum neurotoxins among which the principal prevalent types in humans include A, B and E. Infant botulism results from intestinal colonization and toxin production by C. botulinum spores in babies less than 1 year old. Honey is the most important food discriminated...
متن کاملthe investigation of the relationship between type a and type b personalities and quality of translation
چکیده ندارد.
Isolation of Clostridium botulinum from Honey.
Methods for the isolation of Clostridium botulinum from honey samples are described. A total of 9 of 90 honey samples were positive for C. botulinum; 6 of the positive samples had been fed to babies who developed infant botulism.
متن کاملCloning, Expression and Purification of Clostridium botulinum Neurotoxin Type E Binding Domain
متن کامل
Immunogenic and Protective Potentials of Recombinant Receptor Binding Domain and a C-Terminal Fragment of Clostridium botulinum Neurotoxin Type E
Clostridium Botulinum Type E neurotoxin heavy chain consists of two domains: the translocation domain asthe N-terminal half and the binding domain as the Cterminal half (Hc). One effective way to neutralize botulinum neurotoxin is to inhibit binding of this toxin to neuromuscular synapses with antibodies against binding domain. Two synthetic genes, coding for Hc (the full length binding d...
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عنوان ژورنال:
journal of agricultural science and technologyجلد ۱۹، شماره ۲، صفحات ۳۳۵-۳۴۳
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